Methods for Studying Tubular Transport of Organic Substances

摘要: Advances in knowledge of the mechanisms by which renal tubules transport organic compounds can hardly be separated from advances methods to study function. Almost every new technical approach sooner or later has led quantitative information and often concepts regarding handling substances. Thus, with introduction clearance techniques came description reabsorptive secretory capacity whole kidney for a large number [1], as well conceptual development carrier-mediated tubular processes [1]. Micropuncture studies [2,3,4], became possible only chemical ultramicroanalysis, have demonstrated that different nephron segments solutes do so not at rates but even directions across wall. The polarity cellular was fully appreciated after method microperfusion vivo vitro had become available, technique particularly useful arrive kinetic characterizations processes.