Distribution of Mutations Associated with Antifolate and Chloroquine Resistance among Imported Plasmodium vivax in the State of Qatar.

摘要: Plasmodium vivax is the most prevalent parasite worldwide, escalating by spread of drug resistance. Currently, in Qatar, chloroquine (CQ) plus primaquine are recommended for treatment P. malaria. The present study examined prevalence mutations dihydrofolate reductase (dhfr), dihydropteroate synthase (dhps) genes and CQ resistance transporter (crt-o) genes, associated with sulphadoxine-pyrimethamine (SP) resistance, among imported cases Qatar. Blood samples were collected from patients positive seeking medical at Hamad General Hospital, Doha, during 2013-2016. Sanger sequencing method was performed to examine single nucleotide polymorphisms Pvdhfr, Pvdhps, Pvcrt-o genes. Of 314 isolates, 247 (78.7%), 294 (93.6%) 261 (83.1%) successfully amplified sequenced Pvcrt-o, respectively. Overall, 53.8% (N = 133) carried mutant alleles (58R/117N) whereas 77.2% 227) 90% 235) isolates possessed wild type allele Pvdhps In addition, a total eleven distinct haplotypes detected Pvdhfr/Pvdhps Interestingly, K10 insertion gene observed only originating Indian subcontinent. results suggested that remains an acceptable regimen but further clinical data required assess effectiveness SP Qatar support current national guidelines. limited distribution genetic infections, necessitates regular monitoring resistant malaria