Serotonin-2C receptor involved serotonin-induced Ca 2+ mobilisations in neuronal progenitors and neurons in rat suprachiasmatic nucleus
作者： Kouhei Takeuchi , Shahid Mohammad , Tomoya Ozaki , Eri Morioka , Kaori Kawaguchi
DOI: 10.1038/SREP04106
关键词:
摘要: The hypothalamic suprachiasmatic nucleus (SCN), the central circadian pacemaker in mammals, undergoes serotonergic regulation, but underlying mechanisms remain obscure. Here, we generated a subclone of an SCN progenitor cell line expressing Ca2+ sensors (SCN2.2YC) and compared its 5-HT receptor signalling with that rat neurons brain slices. SCN2.2YC cells expressed 5-HT1A/2A/2B/2C, not 5A/7, while all six subtypes were tissues. High K+ or increased cytosolic cells. responses inhibited by ritanserin SB-221284, resistant to WAY-100635 RS-127445, suggesting predominant involvement 5-HT2C for mobilisations. Consistently, imaging voltage-clamp electrophysiology using slices demonstrated post-synaptic expression. Because expression was postnatally 5-HT-induced mobilisations amplified differentiated developed neurons, suggest this development occurs accordance clock maturations.
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