Gastroprotective effect of Neem (Azadirachta indica) bark extract: Possible involvement of H+-K+-ATPase inhibition and scavenging of hydroxyl radical

摘要: The antisecretory and antiulcer effects of aqueous extract Neem (Azadirachta indica) bark have been studied along with its mechanism action, standardisation safety evaluation. can dose dependently inhibit pylorus-ligation drug (mercaptomethylimidazole)-induced acid secretion ED(50) value 2.7 2 mg Kg(-1) b.w. respectively. It is highly potent in dose-dependently blocking gastric ulcer induced by restraint-cold stress indomethacin 1.5 1.25 When compared, equipotent to ranitidine but more than omeprazole inhibiting secretion. In a model, it effective almost omeprazole. Bark inhibits H(+)-K(+)-ATPase activity vitro concentration dependent manner similar offers gastroprotection against significantly preventing adhered mucus endogenous glutathione depletion. prevents oxidative damage the mucosa lipid peroxidation scavenging hydroxyl radical ((z.rad;)OH)-the major causative factor for ulcer. (z.rad;)OH-mediated human mucosal DNA also protected vitro. melatonin, vitamin E, desferrioxamine alpha-phenyl N-tert butylnitrone, known antioxidants having effect. Standardisation bioactive high pressure liquid chromatography indicates that peak 1 chromatogram coincides compound, phenolic glycoside, isolated from extract. pharmacological are attributed glycoside which apparently homogeneous HPLC represents 10% raw A single 1g per kg (mice) given one day application 0.6g oral route over 15 days cumulative 9g was well tolerated below LD(50). rats no significant adverse concluded has therapeutic potential control hyperacidity