Allergens of the house dust mite Dermatophagoides farinae—Immunochemical studies of four allergenic fractions
作者： M HAIDA , H OKUDAIRA , T OGITA , K ITO , T MIYAMOTO
DOI: 10.1016/0091-6749(85)90094-6
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摘要: Analysis of the allergenic components house dust mite Dermatophagoides farinae was performed. Four different types proteins reactive with human anti-mite IgE antibodies were identified as four distinct radioprecipitins in radioimmunoelectrophoresis. These designated allergen (Me) Me 1, 2, 3, and 4 according to their electrophoretic mobility from slower faster. 1 2 first second prevailing allergens, respectively, 133 atopic patients subjected study. on purification by gel filtration anion exchange chromatography, elicited a single radioprecipitin also band sodium dodecyl sulphate-polyacrylamide electrophoresis peak high-performance liquid chromatography. Both methods identical values 17,000 daltons for molecular weight 1. The isoelectric point suggested be 8.0. higher skin reactions compared that obtained crude extract when it assessed seven serial titration intradermal testing.
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