Iron loading and erythrophagocytosis increase ferroportin 1 (FPN1) expression in J774 macrophages.

摘要: The expression of ferroportin1 (FPN1) in reticuloendothelial macrophages supports the hypothesis that this iron-export protein participates iron recycling from senescent erythrocytes. To gain insight into FPN1's role macrophage metabolism, we examined effect status and erythrophagocytosis on FPN1 J774 macrophages. Northern analysis indicated mRNA levels decreased with depletion increased loading. iron-induced induction was blocked by actinomycin D, suggesting transcriptional control responsible for effect. After erythrophagocytosis, were also up-regulated, increasing 8-fold after 4 hours returning to basal 16 hours. Western corresponding increases levels, maximal 10 Iron chelation suppressed results erythrocyte-derived iron. Comparative analyses iron-related genes revealed a 16-fold increase 3 hours, 10-fold heme oxygenase-1 (HO-1) 2-fold natural resistance macrophage-associated 1 (Nramp1) 6 but no change divalent metal ion transporter (DMT1) levels. rapid strong suggests plays recycling.