Antioxidant supplementation to medium for in vitro embryo production in Felis catus.
作者： N. Cocchia , L. Avallone , S. Tafuri , F. Ciani , L. Esposito
DOI: 10.24425/PJVS.2019.129966
关键词:
摘要: The development of in vitro embryo production (IVEP) techniques Felis catus is a fitting model with potential application to the conservation endangered felid species. To improve quality IVEP an appropriate balance pro- and antioxidants should be provided. Under conditions, high levels superoxide dismutase (SOD), glutathione peroxidase (GPx) catalase (CAT) mRNA provide defence mechanism against oxidative stress for embryos. In order cat oocytes, effects SOD CAT supplemented maturation (IVM) medium GPx fertilization (IVF) on were evaluated. Data showed increase 70 77 % cleaved blastocyst formation, respectively, experiment addition IVM medium; IVF number embryos doubled increased by 96 %. Therefore, our results positive encourage us continue studies oocytes evaluating various dosages combination antioxidants.
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