B cell development in mice that lack one or both immunoglobulin kappa light chain genes.

摘要: We have generated mice that lack the ability to produce immunoglobulin (Ig) kappa light chains by targeted deletion of J and C gene segments intervening sequences in mouse embryonic stem cells. In wild type mice, approximately 95% B cells express only 5% lambda chains. Mice heterozygous for 2-fold more lambda+ than wild-type littermates. Compared with normal homozygous mutants about half number both newly peripheral cell compartments, all these their Ig. Therefore, mutant appear lambda-expressing at nearly 10 times rate observed mice. These findings demonstrate assembly and/or expression is not a prerequisite expression. Furthermore, there no detectable rearrangement 3' RS thus rearrangements sequences, per se, required chain assembly. discuss context implications control Ig potential applications animals.