Systematic comparison of biologically active foreign ions-codoped calcium phosphate microparticles on osteogenic differentiation in rat osteoporotic and normal mesenchymal stem cells.

摘要: // Xiao-Yi Chen 1, * , San-Zhong Xu 2, Xuan-Wei Wang Xian-Yan Yang 3 Liang Ma Lei Zhang 4 Guo-Jing Fan 1 Lin-Hong Xin-Li 5 Kang Ting Chang-You Gao Xiao-Zhou Mou Zhong-Ru Gou and Hai Zou 6 Clinical Research Institute, Zhejiang Provincial People’s Hospital, Hangzhou 310014, China 2 The First Affiliated School of Medicine University, 310009, Zhejiang-California International Nanosystems 310058, Rui’an Hospital & 3rd to Wenzhou Medical 325005, Dental Craniofacial Dentistry, University California, Los Angeles, CA 90095, USA Department Cardiology, These authors have contributed equally this work Correspondence to: Mou, email: mouxz@zjheart.com Gou, zhrgou@zjheart.com Zou, haire1993@163.com Keywords: mineral micronutrients, microparticles, osteogenic differentiation, rOMSCs, rMSCs Received: January 02, 2017     Accepted: March 06, Published: 28, 2017 ABSTRACT Osteoporosis is a disease characterized by structural deterioration bone tissue, leading skeletal fragility with increased fracture risk. Calcium phosphates (CaPs) are widely used in tissue engineering strategies as they similarities apatite except for the absence trace elements (TEs) CaPs. Bioactive glasses (BGs) also been successfully clinic craniomaxillofacial dental applications during last two decades due their excellent potential bonding inducing osteoblastic differentiation. In study, we evaluated effects ionic dissolution products quaternary Si-Sr-Zn-Mg-codoped CaP (TEs-CaP) or 45S5 Bioglass® (45S5 BG), both mixtures separately, on rat marrow-derived mesenchymal stem cells (rOMSCs rMSCs) from osteoporotic normal animals, using an MTT test Alizarin Red S staining. materials enhanced cell proliferation especially combination BG TEs-CaP. Analysis quantitative PCR ELISA indicated that expression osteogenic-specific genes proteins were elevated. investigations suggest TEs-CaP operate synergistically create extracellular environment promotes terminal differentiation rMSCs.