Specific glycoprotein antigens on the surface of insect and mammalian stages of Trypanosoma cruzi

摘要: Two major surface antigens on Trypanosoma cruzi, the causative agent of Chagas disease, have been described [Nogueira, N., Chaplan, S., Tydings, J., Unkeless, J. & Cohn, Z. (1981) Exp. Med. 153, 629-639]. One, a Mr 75,000 glycoprotein (GP), is specific for culture forms (insect-host stages) organisms--epimastigotes and metacyclic trypomastigotes. The other, 90,000 GP, was found in vertebrate-host stages organisms--bloodstream-form We now report that these two T. cruzi seem to be unrelated proteins, as judged by tryptic chymotryptic peptide analysis. Antibodies were raised rabbits against epimastigote or trypomastigote proteins which had immunoprecipitated with human antisera. These protein antisera reacted only homologous immunogen, determined immunoprecipitation surface-labeled organisms immunofluorescence. GP detected cultured (insect stage) epimastigotes present bloodstream-form trypomastigotes, amastigotes, trypomastigotes obtained from infected muscle cells vitro. Therefore, insect vertebrate this species display distinctive GPs can identified labeling techniques six strains (Y, CL, Peru, Colombiana, SF-12, SF-21) isolated widely different areas South America.