MS/MS simplification by 355 nm ultraviolet photodissociation of chromophore-derivatized peptides in a quadrupole ion trap.
作者： Jeffrey J. Wilson , Jennifer S. Brodbelt
DOI: 10.1021/AC071241T
关键词:
摘要: Ultraviolet photodissociation (UVPD) of chromophore-modified peptides enhances the capabilities for de novo sequencing in a quadrupole ion trap mass spectrometer. Attachment UV chromophores allows efficient photoactivation not only precursor ions but also any fragments that retain chromophore functionality. For doubly protonated peptides, UVPD leads to vast reduction MS/MS complexity. The array b and y typically seen upon collisionally activated dissociation is reduced single series either or by depending on location (i.e., N- C-terminus). sulfonation reagent Alexa Fluor 350 (AF350) provided best overall results singly charged UVPD. nonsulfonated analogue AF350, 7-amino-4-methylcoumarin-3-acetic acid, led simplified spectra charged, Dinitrophenyl-peptides yielded albeit with small amount in...
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