Mammalian Cell Biotechnology in Protein Production

摘要: Part 1 Genetic manipulation of mammalian cells: Heterologous expression genes in cells, Hauser Permanent gene cells - transfer and selection, Schlokar Vector for animal Sandig et al Aspects amplification recombinant Wurm Isolation cell clones exhibiting high-level the introduced gene, Wirth engineering antibodies derivatives from cells,Weidle Safety evaluation products derived lines, Onions. 2 Biological aspects Metabolic control culture processes, Wagner Glycosylation a post-translational modification, Savage. 3 Cell cultivation technology: Bioreactors designed Tokashiki, Yokoyama Hydrodinamic properties bioreactors, Chattopadhyay Kinetics simulation Goergen On-line Off-line process analysis, Scheper al. 4 Down-stream processing: Principles product extraction purification pharmaceutical proteins, Walter, Allgaier.