Changes in platelet morphology and function during 24 hours of storage
作者： S. Braune , M. Walter , F. Schulze , A. Lendlein , F. Jung
DOI: 10.3233/CH-141876
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摘要: Abstract For in vitro studies assessing the interaction of platelets with implant materials, common and standardized protocols for preparation platelet rich plasma (PRP) are lacking, which may lead to non-matching results due diversity applied protocols. Particularly, aging during prolonged storage times is discussed an underestimation material thrombogenicity. Here, we study influence whole blood- PRP-storage on changes morphology function. Blood from apparently healthy subjects was collected according a protocol examined immediately after blood collection, four hours twenty later. The capability adhere form stable aggregates (PFA100, closure time) sodium citrate anticoagulated (WB) using agonists equine type I collagen epinephrine bitartrate (collagen/epinephrine) as well adenosine-5'-diphosphate (collagen/ADP). Circulating were quantified at each time point. Morphology visualized microscopically measured electric field multi-channel counting system (CASY). percentage activated assessed by means P-selectin (CD62P) expression circulating platelets. Furthermore, factor 4 (PF4) release poor (PPP) Whole PFA100 increased stimulation collagen/ADP collagen/epinephrine. Twenty both parameters pathologically above upper limit reference range. Numbers platelets, PRP, decreased hours, but no longer hours. Mean volumes (MPV) large cell ratios (P-LCR, 12 fL - 40 fL) over time. Immediately debris or could be microscopically. After first very small occurred. 24 also progressively increased. In accordance this, CASY revealed increase (up 90 μm diameter) increasing CD62P positive PF4 significantly resting PRP. When soluble ADP added stored PRP samples, number activatable present reveals importance consequent standardization WB Platelet function, particularly reactivity adherent their surrounding milieu, changed rapidly outside vascular system. This knowledge crucial interest, biomaterial development cardiovascular applications, help define standards hemocompatibility testing biomaterials.
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researchgate.net 参考文章(34)
Toni Lindl, Birgit Lewandowski, Sonya Schreyögg, Andrea Stäudte, An evaluation of the in vitro cytotoxicities of 50 chemicals by using an electrical current exclusion method versus the neutral red uptake and MTT assays. Atla-alternatives To Laboratory Animals. ,vol. 33, pp. 591- 601 ,(2005) , 10.1177/026119290503300614
F. Jung, S. Braune, A. Lendlein, Haemocompatibility testing of biomaterials using human platelets. Clinical Hemorheology and Microcirculation. ,vol. 53, pp. 97- 115 ,(2013) , 10.3233/CH-2012-1579
Steffen Braune, Maik von Ruesten-Lange, Christof Mrowietz, Karola Lützow, Toralf Roch, Axel T. Neffe, Andreas Lendlein, Friedrich Jung, Dynamic in vitro hemocompatibility testing of poly(ether imide) membranes functionalized with linear, methylated oligoglycerol and oligo(ethylene glycol). Clinical Hemorheology and Microcirculation. ,vol. 54, pp. 235- 248 ,(2013) , 10.3233/CH-131729
W.H. Reinhart, Platelets in vascular disease Clinical Hemorheology and Microcirculation. ,vol. 53, pp. 71- 79 ,(2013) , 10.3233/CH-2012-1577
Pia R.M. Siljander, Platelet-derived microparticles - an updated perspective. Thrombosis Research. ,vol. 127, ,(2011) , 10.1016/S0049-3848(10)70152-3
J.F. Martin, E.A. Trowbridge, Geraldine Salmon, Julie Plumb, The biological significance of platelet volume: Its relationship to bleeding time, platelet thromboxane B2 production and megakaryocyte nuclear DNA concentration Thrombosis Research. ,vol. 32, pp. 443- 460 ,(1983) , 10.1016/0049-3848(83)90255-4
Friedrich Jung, Christian Wischke, Andreas Lendlein, Degradable, Multifunctional Cardiovascular Implants: Challenges and Hurdles Mrs Bulletin. ,vol. 35, pp. 607- 613 ,(2010) , 10.1557/MRS2010.529
I. Mackie, P. Cooper, A. Lawrie, S. Kitchen, E. Gray, M. Laffan, , Guidelines on the laboratory aspects of assays used in haemostasis and thrombosis International Journal of Laboratory Hematology. ,vol. 35, pp. 1- 13 ,(2013) , 10.1111/IJLH.12004
Jürgen Koscielny, Tunay Aslan, Oliver Meyer, Holger Kiesewetter, Friedrich Jung, Christof Mrowietz, Reinhard Latza, Use of the Platelet Reactivity Index by Grotemeyer, Platelet Function Analyzer, and Retention Test Homburg To Monitor Therapy with Antiplatelet Drugs Seminars in Thrombosis and Hemostasis. ,vol. 31, pp. 464- 469 ,(2005) , 10.1055/S-2005-916682
Panayiotis G. Vlachoyiannopoulos, Marina P. Sikara, John G. Routsias, Martina Samiotaki, George Panayotou, Haralampos M. Moutsopoulos, β2 Glycoprotein I (β2GPI) binds platelet factor 4 (PF4): implications for the pathogenesis of antiphospholipid syndrome Blood. ,vol. 115, pp. 713- 723 ,(2010) , 10.1182/BLOOD-2009-03-206367