Unusually large interferon-α-like mRNAs and high expression of interleukin-6 in human fetal annexes

作者: P Duc-Goiran , C Chany , J Doly

DOI: 10.1016/S0021-9258(19)84735-X

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摘要: Abstract Endogenous interferons (IFNs) with antiviral activity have been detected in human fetal annexes, without any apparent induction. Some are of unusually high molecular weight and antigenic properties (Duc-Goiran P., Robert-Galliot, B., Lopez, J., Chany, C. (1985) Proc. Natl. Acad. Sci. U.S.A. 82, 5010-5014). We study here the expression IFN genes from three antigenically distinct groups, placental tissues taken during third-trimester pregnancies. show by Northern blot analysis RNA probes that IFN-alpha-like transcripts correlate presence functionally active protein, purified immunoaffinity chromatography. fractionate on a formamide sucrose gradient, identify, characterize large 4.3-kb transcript, which may be specifically expressed development. Despite absence IFN-beta protein an was neutralized polyclonal anti-IFN-beta serum placenta could related to beta-like IFN. Besides IFNs, various growth factors cytokines can found placenta. report interleukin-6 at levels annexes. The coexpression Interleukin-6 endogenous IFNs suggests they play important role

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