Analysis of the quasi-steady-state approximation for an enzymatic one-substrate reaction.

摘要: Abstract The validity of the quasi-steady state approximation for calculation rate function an isolated enzyme reaction is analysed by a detailed consideration time dependent process. For characterization deviations real motion from quasi-stationary three kinds error functions are used, relaxation deficit, relative and error. An improved procedure developed to calculate transient states system. maximum distance original estimated general method. By different substrate concentrations as well kinetic constants those parameter regions have been determined, where errors do not exceed tolerated values. It suggested how methods can be applied metabolic pathways.