GC-rich murine adenosine deaminase gene promoter supports diverse tissue-specific gene expression.

作者: Sikha Rauth , Keh-Gang Yang , Anita M. Seibold , Diane E. Ingolia , Susan R. Ross

DOI: 10.1007/BF01233043

关键词:

摘要: The murine adenosine deaminase (ADA) gene has a GC-rich promoter that is structurally typical of many mammalian “housekeeping” promoters. ability theADA to support diverse tissue-specific expression was investigated EndogenousADA in different mouse tissues found vary over >3000-fold range highly complex pattern. This also observed cultured human cell lines derived from tissues. ADA levels all and examined correlated closely with steady-state mRNA levels. Several the showed stage-specific variation during postnatal development. In order determine whether tissue-specificADA controlled by cis-acting sequences upstream coding region, constructs containing reporter regulated gene's 5′ flanking were used generate transgenic mice. All transgene-expressing mice obtained analyzed. Our results demonstrate both vivo context an integrated transgene this can animal.

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