FAK alternative splice mRNA variants expression pattern in colorectal cancer.

摘要: The Focal adhesion kinase (FAK) is a ubiquitous cytoplasmic tyrosine-kinase promoting tumor progression and metastasis processes by acting in cancer cells their microenvironment partners. FAK overexpression primary colon tumors associated to poor colorectal (CRC) patients' outcome. Eight mRNA alternative splice variants have been described contribute additional level of activity regulation, some them corresponding overactivated isoforms. To date, expression implication CRC remain unknown. Here, using different human lines displaying differential invasive capacities an vivo murine model recapitulating the steps development from liver lung metastasis, we identified three out eight (namely FAK0 , FAK28 FAK6 ) differentially expressed along process sites. Our results highlight association between expressions metastatic potential most aggressive cell HT29 HCT116, suggesting that could represent aggressiveness markers CRC. findings also suggest more specific role for interactions microenvironment. In conclusion, targeting common form FAK, might not be good strategy based on numerous roles this physiological processes. contrast, or variants, protein isoforms, may putatively future therapeutic target candidates metastasis.