The activity against leukocyte eicosanoid generation of essential oil and polar fractions of Adesmia boronioides Hook.f.

摘要: The polar fractions (methanol and aqueous extract) essential oil of Adesmia boronioides aerial parts were tested in vitro at concentrations 15 50 microg/mL, for their effects on the COX 5-LOX pathways eicosanoid generation (TXB2, PGE2 LTB4) stimulated rat peritoneal leukocytes. Potent inhibition LTB4 was displayed by methanol extract oil, whereas essentially inactive. also caused potent TXB2 but much less active. production striking, implying that main effect is thromboxane synthetase rather than COX. Although LDH release leukocytes treated concurrently with ionophore, suggesting substantial toxicity to cells, this did not affect cell viability according MTT test when incubated cells absence ionophore. two other extracts tested. It concluded contains substance(s) preferentially inhibit 5-lipoxygenase activity arachidonic acid metabolism suggest may contribute antiinflammatory actions plant.