Adeno-associated-virus Rep sequences, vectors and viruses
作者： Wadie F. Bahou , Patrick Hearing , Varsha Sitaraman
DOI:
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摘要: The invention provides adeno-associated virus (AAV) replication (Rep) sequences. In one embodiment, the nucleotide sequences encoding a chimeric protein, wherein encoded protein contains wild type AAV Rep inhibitory amino acid sequence, and contain scrambled and/or deoptimized polynucleotide sequence sequence. vectors, cells, viruses containing invention's Also provided are methods for detecting portions of which reduce infection productive by viruses. compositions useful site-specific integration expression heterologous recombinant (rAAV) vectors rAAV particles, such as hybrid (e.g., Ad-AAV) comprising vectors. find application in, example, gene therapy vaccines.
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