Fumigation with dazomet modifies soil microbiota in apple orchards affected by replant disease

摘要: Abstract Apple replant disease (ARD) is a disorder that affects apple trees when they are replanted in soil where the same species was previously grown. ARD has been known for long time, but precise cause not yet identified. Although most probably due to combination of abiotic and biotic factors, fact fumigation commonly prevents symptoms, at least temporarily, supports hypothesis microorganisms play an important role it. In order find possible relations between taxa composition microbial communities plant growth ARD-affected orchards, we compared fumigated (dazomet 99%) untreated soils by using next-generation sequencing (NGS) technologies. Soil sampling carried out difference plots became significant terms shoot fruit yield specifically end second growing season. Total DNA extracted two target regions (ITS fungi 16S rDNA bacteria), were pyrosequenced with Roche’s 454 Platform. Both bacterial fungal differed significantly our study. Bacillus sp. (ρ = 0.64), Streptomyces Pseudomonas (ρ = 0.59), Chaetomium (ρ = 0.85) some positively correlated asymptomatic trees. cause-effect relation cannot be proven, results confirm that, dazomet reduces also modifies length, particular increasing presence beneficial their action against pathogens.