Genetic and morphology analysis among the pentaploid F1 hybrid fishes (Schizothorax wangchiachii ♀ × Percocypris pingi ♂) and their parents.

摘要: Triploid and pentaploid breeding is of great importance in agricultural production, but it not always easy to obtain double ploidy parents. However, fishes, chromosome diversiform, which may provide natural parental resources for triploid breeding. Both tetraploid hexaploid exist Schizothorax were thought belong different subfamilies with Percocypris fishes morphology, they are sister genera molecule. Fortunately, the hybrid have been successfully obtained by hybridization wangchiachii (♀, 2n = 6X 148) × pingi (♂, 4X 98). To understand genetic morphological difference among their parents, four methods used this study: karyotype, red blood cell ( RBC ) DNA content determination inter-simple sequence repeat ISSR ). In steady, between parents no obvious preference. The numbers P. reported as 98. study, karyotype S. was 148 36m + 34sm 12st 66t, while that 5X 123 39m 28sm 5st 51t. Similarly, intermediate ISSR, within-group diversity higher than Moreover, distance S.wangchiachii closely related ploidy, suggesting material stably coexisted fishes. This first report show a stable F 1 hybrids produced aquaculture.