Non-invasive sampling in Itatiaia National Park, Brazil: wild mammal parasite detection

摘要: Non-invasive sampling through faecal collection is one of the most cost-effective alternatives for monitoring free-living wild mammals, as it provides information on animal taxonomy well dynamics gastrointestinal parasites that potentially infect these animals. In this context, study aimed to perform an epidemiological survey using non-invasive samples from carnivores and artiodactyls identified by stool macroscopy, guard hair morphology DNA sequencing in Itatiaia National Park. Between 2017 2018, faeces were collected along trails park. The host species macroscopic trichological examinations molecular biology. To investigate parasites, Faust, Lutz modified Ritchie Sheather techniques enzyme immunoassays detect Cryptosporidium sp. antigens used. A total 244 collected. Chrysocyon brachyurus, Leopardus guttulus, Canis familiaris, Cerdocyon thous, Puma yagouaroundi, pardalis, concolor Sus scrofa. There 81.1% positive distributed mainly high part Helminths, especially eggs family Ascarididae, more frequently detected carnivore (70.9%). Protozoa, sp., represented highest frequency infection artiodactyl (87.1%). This zoonotic protozoon was eight mammalian species, including a boar. High values structural richness Shannon Simpson diversity indices observed C. brachyurus. Significant differences parasite between domestic animals, such brachyurus respectively, taxonomically distant S. similarity found among frequented similar areas park, L. guttulus. animals infections combination three techniques. structures diagnosed. Zoonotic protozoa occurred introduced species.