Differential expression of cytokeratin mRNA and protein in normal prostate, prostatic intraepithelial neoplasia, and invasive carcinoma.

摘要: The expression of cytokeratin (CK) mRNA for CK5, -8, -14, -16, and -19 was investigated in normal prostate, prostatic intraepithelial neoplasia (PIN) lesions, invasive carcinoma using situ hybridization. Protein localization carried out adjacent sections immunohistochemistry correlated with expression. Snap-frozen human prostate samples including 22 examples glands, 20 cases PIN 12 were examined. CK5 -14 protein prominently expressed only the basal cells glands lesions. CK14 absent luminal most lesions but seen at a low level some not detected any lesion, suggesting that, if cell that makes up is derived from cell, translation depressed although may persist. CK8 constitutively all epithelia abnormal tissues. CK19 persistently both tubular portion as well heterogeneously alveoli. CK16 similar pattern CK19, either or In conclusion, to its would support an origin this structure rather than alveolar glands. between further supports concept precursor lesion carcinoma.