Development of EST-SSR markers and their application in an analysis of the genetic diversity of the endangered species Magnolia sinostellata.

摘要: Magnolia sinostellata is an endemic species of Magnoliaceae that narrowly distributed in the south Zhejiang Province, China. To explore genetic diversity and population structure this endangered species, study developed sequence tag-simple repeat (EST-SSR) markers based on transcriptome data M. sinostellata. In total, 25472 SSRs were identified among 110644 unique assembled sequences with a total 90.83 Mb average frequency 23.02%. The mononucleotide (33.53%) dinucleotide (42.08%) motifs appeared to be most abundant. 150 potential loci randomly selected validate quality SSR markers; effective PCR rate 32.00% polymorphism 15.33% obtained for these loci. After performing sequencing cloning validation, 23 pairs primers retained used characterize Overall, 204 alleles amplified. results Shannon’s information index (I), heterozygosity (Ho), (He) Nei’s expected (H) indicated rich However, high inbreeding coefficient differential suggest serious drift occurred within populations, differentiation apparent populations. Consequently, although has it still dangerous condition. Habitat destruction caused by human activities main threat enhancing abundance adopting some conservation measures should favourable saving species.