Blood-Derived DNA Methylation Signatures of Crohn's Disease and Severity of Intestinal Inflammation

摘要: Background & Aims Crohn's disease is a relapsing and remitting inflammatory disorder with variable clinical course. Although most patients present an phenotype (B1), approximately 20% of rapidly progress to complicated disease, which includes stricturing (B2), within 5 years. We analyzed DNA methylation patterns in blood samples pediatric at diagnosis later time points identify changes that associate might contribute development progression. Methods obtained from 164 (1–17 years old) (B1 or B2) who participated North American study were followed for Participants without intestinal inflammation symptoms served as controls (n = 74). collected 1–3 850,000 sites. used genetic association the concept Mendelian randomization result disease. Results identified 1189 5′-cytosine–phosphate–guanosine-3′ (CpG) sites differentially methylated between (at diagnosis) controls. Methylation these correlated plasma levels C-reactive protein. A comparison profiles vs during follow-up period showed that, treatment, alterations more closely resembled observed controls, irrespective progression B2. 3 CpG Most associated disappeared treatment be Conclusions accompany acute inflammation; change resemble inflammation. These findings indicate disease–associated are features less likely