Pol κ: A DNA Polymerase Required for Sister Chromatid Cohesion
作者： Zhenghe Wang , Irene B Castano , Alejandro De Las Penas , Carrie Adams , Michael F Christman
DOI: 10.1126/SCIENCE.289.5480.774
关键词:
摘要: Establishment of cohesion between sister chromatids is coupled to replication fork passage through an unknown mechanism. Here we report that TRF4 , evolutionarily conserved gene necessary for chromosome segregation, encodes a DNA polymerase with β-polymerase–like properties. A double mutant in the redundant homologs, and TRF5 unable complete S phase, whereas trf4 single completes presumably defective phase results failure replicated chromatids. This suggests TRF s are key link coordination chromatid cohesion. Trf4 Trf5 represent fourth class essential nuclear polymerases (designated kappa) Saccharomyces cerevisiae probably all eukaryotes.
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