Modulation of non-bilayer lipid phases and the structure and functions of thylakoid membranes: effects on the water-soluble enzyme violaxanthin de-epoxidase
作者： Ondřej Dlouhý , Irena Kurasová , Václav Karlický , Uroš Javornik , Primož Šket
DOI: 10.1038/S41598-020-68854-X
关键词:
摘要: The role of non-bilayer lipids and non-lamellar lipid phases in biological membranes is an enigmatic problem membrane biology. Non-bilayer are present large amounts all membranes; energy-converting they constitute about half their total content-yet functional state a bilayer. In vitro experiments revealed that the functioning water-soluble violaxanthin de-epoxidase (VDE) enzyme plant thylakoids requires presence phase. 31P-NMR spectroscopy has provided evidence on polymorphism thylakoid membranes. Here we reveal reversible pH- temperature-dependent changes lipid-phase behaviour, particularly flexibility isotropic phases, isolated spinach thylakoids. These reorganizations accompanied by permeability thermodynamic parameters appear to control activity VDE photoprotective mechanism non-photochemical quenching chlorophyll-a fluorescence. data demonstrate, for first time native membranes, modulation
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Reimund Goss, Martin Lohr, Dariusz Latowski, Joanna Grzyb, Astrid Vieler, Christian Wilhelm, Kazimierz Strzalka, None, Role of hexagonal structure-forming lipids in diadinoxanthin and violaxanthin solubilization and de-epoxidation Biochemistry. ,vol. 44, pp. 4028- 4036 ,(2005) , 10.1021/BI047464K
Matthew P. Davey, Alison G. Smith, Lipids in photosynthesis. Essential and regulatory functions Annals of Botany. ,vol. 111, ,(2013) , 10.1093/AOB/MCS277
Wolfgang Bilger, Olle Bj�rkman, Role of the xanthophyll cycle in photoprotection elucidated by measurements of light-induced absorbance changes, fluorescence and photosynthesis in leaves of Hedera canariensis. Photosynthesis Research. ,vol. 25, pp. 173- 185 ,(1990) , 10.1007/BF00033159
H. Harańczyk, K. Strzałka, W. Dietrich, J. S. Blicharski, 31P-NMR observation of the temperature and glycerol induced non-lamellar phase formation in wheat thylakoid membranes Journal of Biological Physics. ,vol. 21, pp. 125- 139 ,(1995) , 10.1007/BF00705595
John M. Seddon, Gregor Cevc, Derek Marsh, Calorimetric studies of the gel-fluid (L beta-L alpha) and lamellar-inverted hexagonal (L alpha-HII) phase transitions in dialkyl- and diacylphosphatidylethanolamines. Biochemistry. ,vol. 22, pp. 1280- 1289 ,(1983) , 10.1021/BI00274A045
Harry Y. Yamamoto, Lavonne Kamite, The effects of dithiothreitol on violaxanthin de-epoxidation and absorbance changes in the 500-nm region Biochimica et Biophysica Acta (BBA) - Bioenergetics. ,vol. 267, pp. 538- 543 ,(1972) , 10.1016/0005-2728(72)90182-X
S. J. Singer, G. L. Nicolson, The Fluid Mosaic Model of the Structure of Cell Membranes Science. ,vol. 175, pp. 720- 731 ,(1972) , 10.1126/SCIENCE.175.4023.720
Ben de Kruijff, Lipids beyond the bilayer Nature. ,vol. 386, pp. 129- 130 ,(1997) , 10.1038/386129A0
Richard M Epand, Lipid polymorphism and protein-lipid interactions. Biochimica et Biophysica Acta. ,vol. 1376, pp. 353- 368 ,(1998) , 10.1016/S0304-4157(98)00015-X
Anelia G. Dobrikova, Zsuzsanna Várkonyi, Sashka B. Krumova, László Kovács, Georgi K. Kostov, Svetla J. Todinova, Mira C. Busheva, Stefka G. Taneva, Győző Garab, Structural rearrangements in chloroplast thylakoid membranes revealed by differential scanning calorimetry and circular dichroism spectroscopy. Thermo-optic effect. Biochemistry. ,vol. 42, pp. 11272- 11280 ,(2003) , 10.1021/BI034899J