Influence of Stress Conditions on Bacteroides fragilis Survival and Protein Profiles
作者： Katia Eliane Santos Avelar , Saulo Roni Moraes , Leandro Jose Freire Pinto , Wilenes das Gracas Silva E Souza , Regina Maria Cavalcanti Pilotto Domingues
DOI: 10.1016/S0934-8840(98)80176-X
关键词:
摘要: Summary Bacteroides fragilis strains isolated from different sources, i. e. 1 strain (AA1) an aquatic environment, normal flora (118310) and the type (ATCC 25285) originally clinical material, were analysed for both cell envelope proteins composition surviving under oxidative stress starvation. All examined showed a similar survival response when cultured in drinking water with ten-fold decrease viable counts per day during 7 days of analysis. The outer membrane protein (OMP) profiles all quite period as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). However, periplasmic 118310 two bands at 48 58 kDa, respectively, that absent AA1 ATCC 25285 incubation potable water. Whole cells 35S-labelled bacteria significant increase 16, 18, 24, 26, 35, 48, kDa 22, 58, 70 compared to grown BHI-PRAS media detected autoradiography following SDS-PAGE. These data suggest B. may have synthesis mechanism allows them adapt adverse environments.
sci-hub.se 参考文章(21)
B J Moncla, P Braham, S L Hillier, Sialidase (neuraminidase) activity among gram-negative anaerobic and capnophilic bacteria. Journal of Clinical Microbiology. ,vol. 28, pp. 422- 425 ,(1990) , 10.1128/JCM.28.3.422-425.1990
Rial D. Rolfe, David J. Hentges, Benedict J. Campbell, James T. Barrett, Factors Related to the Oxygen Tolerance of Anaerobic Bacteria Applied and Environmental Microbiology. ,vol. 36, pp. 306- 313 ,(1978) , 10.1128/AEM.36.2.306-313.1978
G F Ames, C Prody, S Kustu, Simple, rapid, and quantitative release of periplasmic proteins by chloroform. Journal of Bacteriology. ,vol. 160, pp. 1181- 1183 ,(1984) , 10.1128/JB.160.3.1181-1183.1984
D S Shoop, L L Myers, J B LeFever, Enumeration of enterotoxigenic Bacteroides fragilis in municipal sewage. Applied and Environmental Microbiology. ,vol. 56, pp. 2243- 2244 ,(1990) , 10.1128/AEM.56.7.2243-2244.1990
Penny S. Amy, Richard Y. Morita, Starvation-Survival Patterns of Sixteen Freshly Isolated Open-Ocean Bacteria † Applied and Environmental Microbiology. ,vol. 45, pp. 1109- 1115 ,(1983) , 10.1128/AEM.45.3.1109-1115.1983
J P Schumann, D T Jones, D R Woods, UV light induction of proteins in Bacteroides fragilis under anaerobic conditions. Journal of Bacteriology. ,vol. 151, pp. 44- 47 ,(1982) , 10.1128/JB.151.1.44-47.1982
T Nyström, R M Olsson, S Kjelleberg, Survival, stress resistance, and alterations in protein expression in the marine vibrio sp. strain S14 during starvation for different individual nutrients. Applied and Environmental Microbiology. ,vol. 58, pp. 55- 65 ,(1992) , 10.1128/AEM.58.1.55-65.1992
Penny S. Amy, Richard Y. Morita, Protein Patterns of Growing and Starved Cells of a Marine Vibrio sp. † Applied and Environmental Microbiology. ,vol. 45, pp. 1748- 1752 ,(1983) , 10.1128/AEM.45.6.1748-1752.1983
M A Hood, G E Ness, Survival of Vibrio cholerae and Escherichia coli in estuarine waters and sediments. Applied and Environmental Microbiology. ,vol. 43, pp. 578- 584 ,(1982) , 10.1128/AEM.43.3.578-584.1982
Regina Maria Cavalcanti Pilotto Domingues, Marcia De Oliveira, Raphael Hirata Junior, Arnaldo Feitosa Braga De Andrade, Maria Candida De Souza Ferreira, Bacteroides fragilis: an exogenous pathogen? Zentralblatt Fur Bakteriologie-international Journal of Medical Microbiology Virology Parasitology and Infectious Diseases. ,vol. 282, pp. 296- 302 ,(1995) , 10.1016/S0934-8840(11)80130-1