Spatiotemporal organization and thalamic modulation of seizures in the mouse medial thalamic-anterior cingulate slice.

摘要: SUMMARY Purpose: Seizure-like activities generated in anterior cingulate cortex (ACC) are usually classified as simple partial and associated with changes autonomic function, motivation, thought. Previous studies have shown that thalamic inputs can modulate ACC seizure, but the exact mechanisms not been studied thoroughly. Therefore, we investigated role of modulating seizure-like activities. In addition, seizure onset propagation difficult to determine vivo ACC. We spatiotemporal epileptiform activity this a thalamic‐ACC slice clearly onset. Methods: used multielectrode array (MEA) recording calcium imaging investigate modulatory effect preparation. Key Findings: induced 4-aminopyridine (4-AP; 250 lM) bicuculline (5‐50 lM )i n were attenuated by glutamate receptor antagonists, degree disinhibition varied dose bicuculline. decreased 1 Hz stimulation, whereas corpus callosum stimulation could increase ictal discharges. Amplitude duration augmented after removing inputs, was observed those elevated (50 lM). initiated layers II/III and, lesions, they occurred mainly V/VI. Two-dimensional current-source density analyses revealed sink signals more frequently V/VI indicating these produce larger excitatory synchronization. Calcium transients synchronized lesions suggesting subjected desynchronizing modulation inputs. subject from medial deep layer pyramidal neurons. Significance: Cingulate modulated significantly Repeated thalamus efficiently inhibited activity, demonstrating desynchronization pathway-specific. The clinical implications epileptic require further investigation.