Genome-wide analysis of A-to-I RNA editing by single-molecule sequencing in Drosophila

摘要: The accurate and thorough genome-wide detection of adenosine-to-inosine editing, a biologically indispensable process, has proven challenging. Here, we present discovery pipeline in adult Drosophila, with 3,581 high-confidence editing sites identified an estimated accuracy 87%. target genes specific highlight global biological properties functions RNA including hitherto-unknown well-characterized classes noncoding RNAs 645 that cause amino acid substitutions, usually at conserved positions. spectrum these gene targets encompass suggests participates diverse set cellular processes. Editing Drosophila exhibit sequence-motif preferences tend to be concentrated within small subset total RNAs. Finally, regulates expression levels mRNAs strongly correlates alternative splicing.