Alpha-lipoic acid preserves skeletal muscle mass in type 2 diabetic OLETF rats

摘要: Increased oxidative stress and impaired antioxidant defense are important mechanisms in the pathogenesis of diabetic myopathy. Alpha-lipoic acid (ALA) has been indicated as a weight-loss treatment rodents humans, but studies limited. In present study, we aimed to determine influence ALA, potent biological antioxidant, on metabolic growth processes rat skeletal muscle. Male 25-week-old type 2 rats (OLETF) were randomly divided into two groups, control group (OLETF-C) an ALA-treated (OLETF-ALA) supplemented with 100 mg/kg ALA for 8 weeks. Age-matched, healthy, nondiabetic LETO (LETO-C) used controls. At 32 weeks age, body weight was decreased by 6.8%, areas under curve IP-GTT, fasting glucose, insulin less OLETF-ALA compared OLETF-C rats. significantly preserved muscle mass enhanced fiber cross-sectional area frequency percentage OLETF Although activation myoD, myogenin, myostatin gastrocnemius inhibited relative rats, there no differences expression levels atrogin-1 MuRF1 between groups. increased phosphorylated 5′-AMPK, SIRT1, PGC-1α, well AKT, mTOR, p70S6 kinase contrast, p38 MAPK, IRS-1, FOXO1 muscles upregulated AMPK/SIRT1/PGC-1α AKT/mTOR/p70S6K signaling pathways Therefore, may be potential therapeutic intervention loss animal models resistance.