Cooperation of C1q Receptors and Integrins in C1q-Mediated Endothelial Cell Adhesion and Spreading

摘要: The interaction of C1q with endothelial cells elicits a multiplicity biologic responses. Although these responses are presumed to be mediated by the cell surface proteins, identity participants is not known. In this study we examined roles two binding cC1q-R/calreticulin and gC1q-R/p33, in C1q-mediated adhesion spreading human dermal microvascular (HDMVEC). When HDMVEC were cultured microtiter plate wells coated concentrations ranging from 0 50 μg/ml, specific dose-dependent was observed. extent similar seen on collagen-coated wells. Spreading (68 ± 12%) moderate (47 9%) inhibited anti-gC1q-R mAb 60.11. Similar effects noted polyclonal anti-cC1q-R but control nonimmune IgG. Abs had slight additive effect (75 13% inhibition) when mixed together proportion 100 μg/ml 30 anti-cC1q-R. More importantly, 100% inhibition spreading, adhesion, C1q-coated observed presence μM peptide GRRGDSP GRRGESP. Furthermore, while anti-β1 integrin Ab blocked both anti-α5 only adhesion. Ag capture ELISA membrane proteins using showed β1 α5 integrins also CD44. Taken results suggest that require cooperation C1qRs possibly other membrane-spanning molecules.