Functional expression of xylose isomerase in flocculating industrial Saccharomyces cerevisiae strain for bioethanol production.

摘要: Saccharomyces cerevisiae strains with xylose isomerase (XI) pathway were constructed using a flocculating industrial strain (YC-8) as the host. Both expressing wild-type xylA (coding XI) from fungus Orpinomyces sp. and bacterium Prevotella ruminicola, respectively, showed better growth ability fermentation capacity when sole sugar than most of reported XI. Codon optimization both XIs did not improve strains. Adaption significantly increased XI activity resulting in improved fermentation. The codon-optimized higher increase consumption ethanol production compared to wild Among all strains, adapted YCPA2E P. ruminicola best performance ethanol. After 48 h fermentation, assimilated 16.95 g/L produced 6.98 g/L These results indicate that YC-8 is suitable host for expression, especially originating P. ruminicola.