Produção de inóculo de Alternaria cassiae
作者： Zila Ribeiro de Avila , SUELI CORRÊA MELLO , ZILDA RIBEIRO , MARIA DE ARAÚJO , ELIANA FONTES
DOI: 10.1590/S0100-204X2000000300008
关键词:
摘要: In order to adjust the methodology of inoculum production, an isolate fungus Alternaria cassiae, selected as a biological control agent for sicklepod (Senna obtusifolia) was studied under different culture media and mycelial growth conditions. Homemade V-8 juice medium without CaCO3 addition, incubation on 200-250 mL (around 50% flask volume) with agitation at 250 rpm during 48 hours, followed by mass in continuous dark were appropriate conditions large scale production inoculum. The sporulation aided 25oC. use 300 per pan (29.9 44.5 5.0 cm ) induce provided rapid dryness ensured conidium integrity, indicated higher infectivity
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