Adenosine dialdehyde and neplanocin A: Potent inhibitors of S-adenosylhomocysteine hydrolase in neuroblastoma N2a cells
作者： Vanitha Ramakrishnan , Ronald T. Borchardt
DOI: 10.1016/0197-0186(87)90068-4
关键词:
摘要: Abstract S-Adenosylhomocysteine hydrolase (AdoHcy hydrolase, E.C. 3.3.1.1) catalyzes the metabolism of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and homocysteine (Hcy) in mouse neuroblastoma N2a cells. AdoHcy cells can be inhibited completely by dialdehyde (Ado dialdehyde) or neplanocin A. The inhibitory effects Ado (2.5 μM) A (1 on cellular were time-dependent, with total enzyme inhibition occurring after 30 min 15 incubation, respectively. produced persisted for up 72 h was paralleled a time-dependent increase endogenous levels reaching maximum 4-fold elevation 8 incubation an 11-fold A-treated This subsequent S-adenosylmethionine (AdoMet)-dependent methylations (e.g. protein carboxylmethylation (PCM), lipid methylation). In addition, metabolically converted corresponding AdoMet analog, S-neplanocylmethionine (NepMet), NepMet reached
sci-hub.se 参考文章(49)
P M Ueland, Pharmacological and biochemical aspects of S-adenosylhomocysteine and S-adenosylhomocysteine hydrolase. Pharmacological Reviews. ,vol. 34, pp. 223- 253 ,(1982)
Melvin L. Billingsley, Randall L. Kincaid, Marina E. Wolf, Robert H. Roth, Walter Lovenberg, Carey D. Balaban, S. Milton, Is There a Function for Protein Carboxylmethylation in the Nervous System Humana Press. pp. 25- 41 ,(1986) , 10.1007/978-1-4612-5012-8_3
Steven Clarke, Protein Methylation at Abnormal Aspartyl Residues Humana Press. pp. 3- 14 ,(1986) , 10.1007/978-1-4612-5012-8_1
Giulio L. Cantoni, Peter K. Chiang, The Role of S-Adenosylhomocysteine and S-Adenosylhomocysteine Hydrolase in the Control of Biological Methylations Natural Sulfur Compounds. pp. 67- 80 ,(1980) , 10.1007/978-1-4613-3045-5_6
Dennis E. Vance, Neale Ridgway, Methylation of Phosphatidylethanolamine: Enzyme Characterization, Regulation and Physiological Function Humana Press. pp. 75- 88 ,(1986) , 10.1007/978-1-4612-5012-8_7
R T Borchardt, J A Huber, A Moorman, D Kuonen, Inhibition of calf thymus and rat hypothalamic synaptosomal protein carboxymethyltransferase by analogues of S-adenosylhomocysteine. Molecular Pharmacology. ,vol. 21, pp. 181- 186 ,(1982)
R T Borchardt, B T Keller, U Patel-Thombre, Neplanocin A. A potent inhibitor of S-adenosylhomocysteine hydrolase and of vaccinia virus multiplication in mouse L929 cells. Journal of Biological Chemistry. ,vol. 259, pp. 4353- 4358 ,(1984) , 10.1016/S0021-9258(17)43053-5
R I Glazer, M C Knode, Neplanocin A. A cyclopentenyl analog of adenosine with specificity for inhibiting RNA methylation. Journal of Biological Chemistry. ,vol. 259, pp. 12964- 12969 ,(1984) , 10.1016/S0021-9258(18)90641-1
D D Flynn, Y Kloog, L T Potter, J Axelrod, Enzymatic methylation of the membrane-bound nicotinic acetylcholine receptor. Journal of Biological Chemistry. ,vol. 257, pp. 9513- 9517 ,(1982) , 10.1016/S0021-9258(18)34100-0
P A Velletri, R H Roth, R J DeLorenzo, M L Billingsley, Carboxylmethylation of calmodulin inhibits calmodulin-dependent phosphorylation in rat brain membranes and cytosol. Journal of Biological Chemistry. ,vol. 258, pp. 5352- 5357 ,(1983) , 10.1016/S0021-9258(20)81895-X