作者: Ronit Machtinger , Catherine Racowsky
DOI: 10.1007/978-1-61779-971-6_12
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摘要: Culture media to support development of zygotes the blastocyst stage is based either on a single medium or sequential (two-step) media. Single culture with without day 3 renewal associated simplified laboratory protocols and lower costs compared medium. There are currently insufficient clinical data conclude that one system, sequential, superior other regard performance. This chapter summarizes rationale for use medium, along supporting animal human culturing embryos in renewed not renewed.