Canine coronavirus infections in Japan: virological and epidemiological aspects.

摘要: Ten strains, eight field and two reference laboratory of canine coronavirus (CCV) were comparatively examined with respect to antigenic relationships pathogenic potential in dogs. With monoclonal antibodies hyperimmune antisera feline CCV, respectively, varying degrees diversities found among the strains by neutralization immunofluorescence assays, but it was felt that they belong one serotype. Specific-pathogen-free puppies experimentally inoculated some CCV manifested clinical symptoms, there a difference their virulence. In order elucidate prevalence infections dogs Japan, we tested for neutralizing 467 dogs, 44.1%. Moreover, using nested reverse transcriptase-polymerase chain reaction on rectal swabs 100 diarrheic recently presented veterinary clinics, evidence 16% these specimens found. The results suggested infection is more widespread than expected significant etiologic factor diarrhea also Japan.