Polychelated cryogels: hemoglobin adsorption from human blood
作者： Kadir Erol
DOI: 10.1080/21691401.2016.1215326
关键词:
摘要: AbstractThe separation and purification methods are extremely important for the hemoglobin (Hb) which is a crucial biomolecule. The adsorption technique popular among these cryogels have been used quite much due to their macropores interconnected flow channels. In this study, Hb onto Cu(II) immobilized poly(2-hydroxyethyl methacrylate-glycidyl methacrylate), poly(HEMA-GMA)-Cu(II), was investigated under different conditions (pH, interaction time, initial concentration, temperature ionic strength) optimize conditions. swelling test, Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscope (SEM), surface area (BET), elemental ICP-OES analysis were performed characterization of cryogels. Polyethyleneimine (PEI) molecule as Cu(II)-chelating ligand. capacity determined 193.8 mg Hb/g cryogel. isolation from human blood also studied optim...
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