Proteomic alterations induced by ionic liquids in Aspergillus nidulans and Neurospora crassa

摘要: Abstract This study constitutes the first attempt to understand at proteomic level fungal response ionic liquid stress. Ascomycota are able grow in media supplemented with high concentrations of an liquid, which, turn, lead major alterations metabolic footprint. Herein, we analysed differential accumulation mycelial proteins Aspergillus nidulans and Neurospora crassa after their exposure two most commonly used liquids: 1-ethyl-3-methylimidazolium chloride or cholinium chloride. Data obtained showed that numerous stress-responsive ( e.g. anti-ROS defence proteins) as well several critical biological processes and/or pathways were affected by either liquid. Amongst other changes, these compounds altered developmental programmes both fungi promoting development Hulle cells conidiation) led osmolytes, some which may play important role multiple stress responses. In particular, N. , liquids increased levels likely involved biosynthesis unusual metabolites. These data potentially open new perspectives on research, furthering conscious design use trigger production targeted Biological significance The present emphasises importance understanding liquid's responses, crucial further safe large-scale usage. Knowledge prompted a cellular biochemical gives also fresh how employ “novel” manipulate biotechnological value. results presented here provide meaningful insights into adaptation responses anthropogenic chemicals industry.