Genome-wide identification and transcriptional characterization of DNA methyltransferases conferring temperature-sensitive male sterility in wheat.

摘要: Background DNA methyltransferase (DMT) genes contribute to plant stress responses and development by de novo establishment subsequent maintenance of methylation during replication. The photoperiod and/or temperature-sensitive genic male sterile (P/TGMS) lines play an important role in hybrid seed production wheat. However, only a few studies have reported on the effect DMT sterility Although been investigated some species, identification analysis wheat (Triticum aestivum L.) based genome-wide levels not reported. Results In this study, detailed overview phylogeny 52 (TaDMT) was presented. Homoeolog retention for TaDMT significantly above average rate whole-wheat genes, indicating functional importance many homoeologs. We found that strikingly high number resulted mainly from significant expansion TaDRM subfamily. Intriguingly, all 5 paralogs belonged DRM subfamily, we speculated tandem duplications might crucial subfamily expansion. Through transcriptional TGMS line BS366 its hybrids with other six fertile under conditions, concluded TaCMT-D2, TaMET1-B1, TaDRM-U6 be involved BS366. Furthermore, correlation showed TaMET1-B1 negatively regulate expression TaRAFTIN1A, gene pollen development, so regarding epigenetic regulatory mechanism underlying via interaction between TaRAFTIN1A. Conclusions Our findings presented phylogenic could provide novel insights into effects