[10] On predicting the results of affinity procedures

摘要: Publisher Summary This chapter illustrates the ligand saturation level to be approximately hyperbolic in concentration of enzyme. Consequently, achieve a relatively high degree gel saturation, enzyme solution should as concentrated possible. Some sacrifice fraction removed from may result, but larger total number moles can loaded on column with solution. Depending one's goal, such manipulation or not desirable. An interesting and important result is that, most cases, very little saturated (typically less than 1% L o ). One aware this equilibrium-based limitation, immediately assume that steric factors are responsible for poor utilization ligand.