The importance of the hydrophilic region of PsbL for the plastoquinone electron acceptor complex of Photosystem II

摘要: Abstract The PsbL protein is a 4.5 kDa subunit at the monomer–monomer interface of Photosystem II (PS II) consisting single membrane-spanning domain and hydrophilic stretch ~ 15 residues facing cytosolic (or stromal) side photosystem. Deletion conserved in N-terminal region has been used to investigate importance this extension. Using Synechocystis sp. PCC 6803, three deletion strains: ∆(N6–N8), ∆(P11–V12) ∆(E13–N15), have created. ∆(N6–N8) strains remained photoautotrophic but were more susceptible photodamage than wild type; however, ∆(E13–N15) cells had most severe phenotype. Δ(E13–N15) mutant showed decreased growth, reduced number PS centers, impaired oxygen evolution presence II-specific electron acceptors, was highly photodamage. decay kinetics chlorophyll variable fluorescence after turnover saturating flash sensitivity low concentrations II-directed herbicides strain indicate that binding plastoquinone Q B -binding site altered such affinity reduced. In addition, acceptor 2,5-dimethyl- p -benzoquinone found inhibit transfer through quinone-acceptor complex strain. Y20A also investigated it exhibited increased susceptibility herbicide sensitivity. Our data suggest influences forward from A indirect interactions with D–E loop D1 reaction center protein. results further disruption between other subunits or lipids destabilizes dimer formation. This article part Special Issue entitled: Photosynthesis Research for Sustainability: Keys Produce Clean Energy.