Combined biocatalytic conversion of smooth hound viscera: Protein hydrolysates elaboration and assessment of their antioxidant, anti-ACE and antibacterial activities

摘要: Abstract The aim of this study was to investigate the antioxidant, anti -ACE and antibacterial activities smooth hound viscera hydrolysates (SHVHs). Viscera, as by-product, were hydrolyzed with crude alkaline protease extract from same species, three commercial proteases a combination endogenous exogenous preparations. Hydrolysates showed different degrees hydrolysis digestion enzymes in found enhance protein hydrolysis. resulting SHVHs had high content (70.89%–89.06%) they mainly constituted by Gly, Glu Gln, while Tau major free amino acid. In addition, contained amounts UMP, Uridine, GMP Guanosine, undigested proteins rich IMP Xanthine. Furthermore, molecular mass distribution RP-HPLC profiles proving their hydrophilic/hydrophobic peptide heterogeneity. All exhibited antioxidant activity, terms radical-scavenging reducing power, metal chelating β-carotene protection, lipid peroxidation inhibition DNA breakage assay, it that medium degree appropriate obtain good capacity. activity improved after simulated gastrointestinal digestion. Besides effect against several Gram-positive Gram-negative bacteria, all varying ACE inhibitory highest one achieved Purafect hydrolysate (IC 50  = 75 μg/mL). overall data suggested could be used potential source natural antimicrobial peptides formulate functional foods.