MarkerMiner 1.0: A New Application for Phylogenetic Marker Development Using Angiosperm Transcriptomes
作者： Srikar Chamala , Nicolás García , Grant T. Godden , Vivek Krishnakumar , Ingrid E. Jordon-Thaden
DOI: 10.3732/APPS.1400115
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摘要: Premise of the study: Targeted sequencing using next-generation (NGS) platforms offers enormous potential for plant systematics by enabling economical acquisition multilocus data sets that can resolve difficult phylogenetic problems. However, because discovery single-copy nuclear (SCN) loci from NGS requires both bioinformatics skills and access to high-performance computing resources, application has been limited. Methods Results: We developed MarkerMiner 1.0, a fully automated, open-access bioinformatic workflow SCN in angiosperms. Our new tool identified as many 1993 transcriptomic sampled part four independent test cases representing marker development projects at different scales. Conclusions: is an easy-to-use effective putative loci. It be run locally or via Web, its tabular alignment outputs facilitate efficient downstrea...
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