Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR

摘要: Real-time reverse transcription followed by polymerase chain reaction (RT–PCR) is the most suitable method for detection and quantification of mRNA. It offers high sensitivity, good reproducibility a wide range. Today, relative expression increasingly used, where target gene standardised non-regulated reference gene. Several mathematical algorithms have been developed to compute an ratio, based on real-time PCR efficiency crossing point deviation unknown sample versus control. But all published equations available models calculation ratio allow only determination single difference between one control sample. Therefore new software tool was established, named REST© (relative tool), which compares two groups, with up 16 data points in group, four genes. The model used efficiencies mean group. Subsequently, results investigated transcripts are tested significance randomisation test. Herein, development application explained usefulness using discussed. latest version examples correct use can be downloaded at http://www.wzw.tum.de/gene-quantification/.