Activities of Some Free-Radical Scavenging Enzymes and Glutathione Concentrations in Human and Rat Liver and their Relationship to the Pathogenesis of Tissue Damage in Iron Overload

摘要: 1. The activities and subcellular distribution of enzymes implicated in the protection cells from free-radical mediated damage were determined liver biopsy specimens control iron-overloaded patients. 2. There was a small but insignificant decrease activity glutathione reductase patients with secondary iron overload due to multiple transfusion therapy for thalassaemia major. 3. superoxide dismutase, catalase peroxidase similar both patient groups. 4. Subcellular fractionation studies indicated major cytosolic localization these minor mitochondrial component. relative proportions two locations 5. Approximately 80% hepatic present reduced form groups it is concluded that although might be pathogenesis tissue no significant defect protective mechanisms can demonstrated.