Adult porcine islets produce MCP-1 and recruit human monocytes in vitro
作者： Cecilia Ehrnfelt , Makiko Kumagai-Braesch , Mehmet Uzunel , Jan Holgersson
DOI: 10.1046/J.1399-3089.2003.00104.X
关键词:
摘要: Type 1 diabetes can be cured by transplantation of isolated pancreatic islets. Because the shortage human donor tissue, adult porcine islets (APIs) constitute a possible alternative tissue source. Upon intraportal injection, are subjected to an instant blood-mediated inflammatory reaction (IBMIR) leading blood clotting, leukocyte islet-infiltration, islet damage and insulin release. Xenogeneic surviving IBMIR rejected in cellular process involving CD4(+) T lymphocytes macrophages. We have investigated whether APIs themselves produce secrete chemokines and/or cytokines that may contribute cell-mediated rejection. APIs, cultured for 1, 4, 8 11 days post-isolation, expressed mRNA monocyte chemoattractant protein-1 (MCP-1), IL-1beta TNF-alpha. API culture supernatants induced migration monocytes, which was significantly blocked anti-human MCP-1 antibody (Ab). Immunohistochemistry revealed cytoplasm alpha- beta-cells situ. However, or their were not able activate aortic endothelial cells (HAECs) vitro, neither nor TNF-alpha detected enzyme-linked immunosorbent assay (ELISA) supernatants. Both recombinant (ECs) inducing CD62E CD106 expression as analyzed flow cytometry. In conclusion, secreted both rejection attracting monocytes into islet; upon transformation macrophages will potentiate antigen presentation execute
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