Enols of aldehydes in the peroxidase/oxidase-promoted generation of excited triplet species.

摘要: General (acid and base) or specific (fluoride ion) catalysis generates the enol of isobutanal propanal from corresponding trimethylsilyl ethers. The enols are directly rapidly oxidized by peroxidase (acting as an oxidase) to triplet acetone acetaldehyde, respectively, formic acid. Due faster rate reaction absence quenching excess aldehyde, excited carbonyl emits more strongly than when aldehyde itself is substrate. With both emission pure phosphorescence. Both acetaldehyde generated within enzyme, shown different D- L-tryptophan, somewhat protected oxygen quenching, attested very fact that phosphorescence observed. use precursors substrates opens wide possibilities for photochemical investigations in light over a much broader range experimental conditions.