MTM1 plays an important role in the regulation of zinc tolerance in Saccharomyces cerevisiae.

摘要: Abstract Background Acquisition and distribution of zinc supports a number biological processes. Various molecular factors are involved in metabolism but not fully explored. Basic procedures Spontaneous mutants were generated yeast with excess culture followed by whole genome DNA sequencing to discover related genes bioinformatics. An identified mutant was characterized through metallomic biology methods. Main findings Here we reported that MTM1 knockout cells displayed much stronger tolerance than wild type on SC medium when exposed zinc. Zn accumulation mtm1Δ dramatically decreased compared under excessive condition due deletion reduced uptake. ZRC1 mRNA level significantly higher the wild-type strain leading increased vacuolar accumulations cells. The levels ZRT1 ZAP1 contributing less zrc1Δmtm1Δ double exhibited sensitivity. did afford resistance an effect mediated influence ROS. Superoxide dismutase 2 (Sod2p) activity severely impaired restored supplementation. Meanwhile, additional showed no significant localization expression Mtm1p. Principal conclusions Our study reveals gene plays important role regulation homeostasis via changing uptake distribution. This discovery provides new insights for better understanding biochemical communication between vacuole mitochondrial relation zinc-metabolism.