Lactoferrin : the role of conformational changes in its iron binding and release

摘要: The relative rates of ferric ion removal from human milk lactoferrin and serum transferrin by the synthetic tricatecholate sequestering agent N,N',N''-tris(5-sulfo-2,3-dihydroxybenzoyl)-1,5,10-triazadecane (3,4-LICAMS) have been compared at pH 7.4 37 o C. Hyperbolic plots observed pseudo-first-order rate constants with increasing concentrations 3,4-LICAMS were obtained for both proteins are consistent a similar mechanism iron this ligand. However, average limiting (k max =6.5(4)×10 -4 min -1 ) is about 100-fold slower than that found =6.3(4)×10 -2 under same conditions